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CD97 Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

CD97 Antibody

貨號(hào)：

HYK-200293
規(guī)格：
圖片：
IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

產(chǎn)品詳情

Uniprot No.：

P48960
基因名：

CD97
別名：

ADGRE5 antibody; Adhesion G protein-coupled receptor E5 antibody; CD 97 antibody; CD_antigen=CD97 antibody; CD97 antibody; CD97 antigen antibody; CD97 antigen subunit beta antibody; CD97 molecule antibody; CD97_HUMAN antibody; Leukocyte antigen CD97 antibody; Seven span transmembrane protein antibody; Seven transmembrane helix receptor antibody; Seven transmembrane heterodimeric receptor associated with inflammation antibody; TM7LN 1 antibody; TM7LN1 antibody
免疫原：

A synthesized peptide
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:500
IF	1:30-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.