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GAPDH Monoclonal Ant...

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GAPDH Monoclonal Antibody

  恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。

  恒遠生物擁有專業的質控團隊,配備先進實驗儀器,現已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標記,大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。

  我們致力于為所有用戶提供高親和力、高特異性的優質抗體。

GAPDH Monoclonal Antibody

 
  • 貨號:
    HYK-200064
  • 規格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
      All lanes GAPDH antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 KDa
      Observed band size: 36 KDa
      Exposure time: 10s

    • Western Blot
      Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse Heart tissue, Mouse lung tissue,Mouse kidney tissue
      All lanes GAPDH antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 KDa
      Observed band size: 36 KDa
      Exposure time: 10s

    • Western Blot
      Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit muscle tissue
      All lanes GAPDH antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 KDa
      Observed band size: 36 KDa
      Exposure time: 10s

    • Western Blot
      Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg All lanes:GAPDH antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 KDa
      Observed band size: 36 KDa
      Exposure time: 10s

    • Western Blot
      Positive WB detected in: 15μg hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 KDa
      Observed band size: 36 KDa
      Exposure time: 10s

    • IHC image of CSB-MA000071M1m diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

    • IHC image of CSB-MA000071M1m diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

    • IHC image of CSB-MA000071M1m diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

    • Immunofluorescence staining of Hela cells with(CSB-MA000071M1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of HepG2 cells with (CSB-MA000071M1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunoprecipitating GAPDH in Hela whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000071M1m in Hela whole cell lysate. Lane 2: CSB-MA000071M1m (5μl) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (10μg)
      For western blotting, the blot was detected with CSB-MA000071M1m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

產品詳情

  • 產品名稱:
    Mouse anti-Homo sapiens (Human) GAPDH Monoclonal Antibody antibody
  • Uniprot No.:
    P04406
  • 基因名:
    GAPDH
  • 別名:
    GAPDH; G3PD; GAPD; MGC88685
  • 宿主:
    Mouse
  • 反應種屬:
    Human, Mouse, Rabbit
  • 免疫原:
    Recombinant Human GAPDH protein (2-335AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal Antibody
  • 抗體亞型:
    IgG1
  • 純化方式:
    >95%, Protein G purified
  • 克隆號:
    10B4E3
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IP, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:5000-1:80000
    IHC 1:200-1:500
    IF 1:50-1:100
    IP 2μl-8μl
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunoprecipitation (IP) Protocol
    Immunofluorescence (IF) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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