Western Blot Positive WB detected in:HepG2 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate All lanes:BCL2 antibody at 1μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa
IHC image of CSB-RA002611A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
產(chǎn)品詳情
產(chǎn)品描述:
BCL2 monoclonal antibody CSB-RA002611A0HU was produced in the rabbit immunized by using the synthesized peptide as the immunogen. The target protein BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. This protein can inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Diseases associated with BCL2 include High Grade B-Cell Lymphoma With Myc And/ Or Bcl2 And/Or Bcl6 Rearrangement and Follicular Lymphoma 1. This BCL2 monoclonal antibody was tested in the ELISA, WB, IHC and FC applications. The non-conjugated IgG got purified by antigen affinity. It reacts with the BCL2 proteins of human or mouse or rat-origin and may be used to detect the endogenous levels of BCL2 protein.
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貨期:
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